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Justice League United (Season 1):

We could say that this season's main antagonist is the White Martian Empire. During the Supergirl (Season 1) Episode 11, the White Martian impersonating Miranda Crane vows there are millions like her ready to kill J'onn and won't rest until he's dead, but Kara tells her she's ready for them. We could say that the Empire planned to invade Earth. During the season, Superman/Kal-El/Clark Kent, Steel/John Henry Irons & Miss Martian/M'gann M'orzz/Megan Morse will join the League.

See Here: Team Roster & Enemies

Justice League United (Season 2):

We could say that this season's main antagonist is T.O.Morrow/Thomas Oscar Morrow & made some improvements on Tornado (made his appearance a bit more heroic), made modifications to his body so he can now assume human appearance with a conscious act of will to infiltrate the D.E.O. 
Red Tornado
while Red Tornado tries to play hero & turn public opinon against Supergirl & created Reds Torpedo & Inferno. A few episodes later, Supergirl & her team figures out that John Smith & R.T. are 1 & the same. Morrow then lured Supergirl into a trap & just completed his new creation "Red Volcano". When Morrow ordered R.T. to kill Supergirl, she reminded him of all the good he's done. Taking these words into mind, R.T. turned on Morrow. After the battle, Supergirl brought a heavily damaged R.T. back to the D.E.O. for repairs. Kara & her team questions Tornado's previous intention, R.T. said: “Morrow created me to be a hero, and he thought that meant less human. To be cold and unfeeling. I wish to be a hero, but I believe that means being more human". From then on, R.T. has served Supergirl & the J.L.U. as a team member. Rocket/Raquel Ervin, Guardian/Jim Harper, Hawkwoman/Shayera Hol/Shiera Hall (who we could've said that she's Kendra Saunders's doppelgänger from Earth-38, only she's a police officer from that universes Thanagar), Red Tornado/John Smith, Maxima (who we could've said that she wishes to turn over a new leaf) & Brainiac 8/Querl Dox would've joined the League.

See Here: Team Roster & Enemies

Justice League United (Season 3):

We could say that this season's main antagonist is Mongul. According to the comics, Mongul is the tyrannical ruler of Warworld, an artificial satellite that traveled the galaxy picking up slaves for gladiatorial combat. We could've said that Mongul had Superman & J'onn kidnapped to have them forced into his gladiatorial combat. Captain Marvel/Billy Batson, Agent Liberty/Ben Lockwood, Icon/Arnus/Augustus Freeman, Insight/Mastrocola, Hawkman/Katar Hol/Carter Hall (who we could've said that he's Carter Hall's doppelgänger from Earth-38, only like his wife, he's a police officer from that universes Thanagar) & Dreamer/Nia Nial would've joined the League.

See Here: Team Roster & Enemies

Justice League United (Season 4):

We could that this season's main antagonist is Despero. According to the comics, Despero is an intergalactic conqueror & the ruthless tyrant ruler of planet Kalanor, he possesses incredible mental powers in addition to his great strength and intellect.

See Here: Team Roster & Enemies

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